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Figure 1.

Detection of NiVsG antibodies in Luminex based multiplexed microsphere assay.

The median fluorescent intensities (MFI) for each microsphere population are shown in graphs. MFI for antibody positive cattle and goat shown in graph I. MFI for antibody positive pig sera is shown in graph II, III and IV. The gray bar represents the detection cut-off of 300 MFI for cattle and goat sera and 650 MFI for pig sera.

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Figure 2.

Western blot analysis against NiV N (nucleocapsid) protein of cattle, goat and pig sera showing higher MFI.

The marker is BenchMark Pre-stained Protein Ladder (Invitrogen); the positive sera (NiV virus neutralization test positive pig and human field sera); the negative control sera (NiV virus neutralization test negative pig).

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Table 1.

NiVsG seropositive animals as detected by Luminex assay.

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Figure 3.

Distribution of NiVsG antibody positive animals in seven districts of Bangladesh.

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Table 2.

NiVsG seropositive animals by district in Bangladesh.

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Table 3.

Demographic characteristics of NiVsG seropositive and seronegative cattle, goats and pigs in Bangladesh.

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Table 4.

Bivariate analysis of feeding practices and environmental exposures associated with NiV serology results in cattle and goats.

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Table 5.

Multivariate logistic regression analysis of feeding practices and environmental exposures associated with NiV serology results in cattle and goats.

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