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Figure 1.

Bayesian phylogram derived from a multilocus analysis of the Triatominae subfamily, includes Zelurus petax and Reduvius personatus from the Reduviinae as outgroup (in blue).

The geographic range for all species modeled in the genus Triatoma is also shown (NCA species are shown in red, South American species in green and from the Antilles in brown). Panstrongylus species are shown in yellow, Mepraia in pink and Rhodnius species are in purple. Branch color indicates PP<0.8 in gray and ≥0.8 in black. Black circles indicate PP≥0.95<; black stars PP≥0.9<0.95.

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Table 1.

Comparative phylogenetic analysis of sister species belonging to the NCA species complexes that were recovered from the Bayesian multilocus and single gene locus analyses.

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Figure 2.

Divergence time estimates for Triatominae clades.

Asterisks and black circles above branches indicate clades supported by PP of 0.9–0.94 and ≥0.95, respectively.

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Figure 3.

Ecological niche models for sister species pairs.

A: Triatoma p. protracta (red) and T. barberi; B: T. rubida (red) and T. nitida (blue); C: T. gerstaeckeri (red) and T. mexicana (blue); D: T. recurva (red) and T. longipennis (blue); E: T. phyllosoma (red) and T. mazzottii (blue); F: T. dimidiata group 2 (red) and T. dimidiata group1a (blue). Grey dots and squares represent the collection sites for each species. Diagonal lines in E and F indicate the overlapping niche range between the sister pairs.

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Figure 4.

Ecological niche similarity tests between sister species pairs of NCA triatomines.

Observed similarity between niches is indicated with the arrows, while bars indicate the null distribution of ecological niche distance generated randomly. Schoeners' D ranges from 0 (complete different ENM), to 1 (identical ENM). In all cases, the observed similarities were higher that their respective null distribution for random niche models.

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