Figure 1.
Characterization of the adult transcriptome, adult proteome, and immunogenic proteins of Paragonimus kellicotti.
Table 1.
Paragonimus kellicotti transcriptome assembly statistics.
Figure 2.
Distribution of protein sequence similarity matches among three trematode species.
Venn diagram showing the distribution of (A) InterPro protein domains and (B) KEGG orthologous groups shared or unique to P. kellicotti, C. sinensis and S. mansoni.
Table 2.
Functions enriched among proteins with predicted secretion peptides.
Table 3.
The top 25 Paragonimus kellicotti proteins in adult worms based on spectral counts.
Figure 3.
Western blot of Paragonimus kellicotti antigen immunoprecipitated with total IgG from P. kellicotti patients.
Total IgG was purified and used to precipitate immunogenic proteins from total P. kellicotti homogenate. P. kellicotti proteins were eluted from the purification column in eight fractions, which were tested by Western blot using an aliquot of the same IgG used in the immunoprecipitation. Fraction 2 had the greatest protein concentration and was used in our mass spectrometry analysis.
Table 4.
The top 25 immunoreactive Paragonimus kellicotti proteins in adult worms based on spectral counts.
Figure 4.
Alignment of myoglobin proteins from Paragonimus species and other trematodes.
Amino acid alignments show 90% sequence identity between myoglobin 1 sequences from P. kellicotti and P. westermani. Far less homology is shared between myoglobins in Paragnomimus and top BLASTP hits from other trematode species. Abbreviations: Pk1, Pk34178_txpt1; Pk2, Pk48549_txpt1; Pw, P. westermani gi:59895953; Cs, C. sinensis gi:349998765; Ov, Opisthorchis viverrini gi: 663047528; Fh, F. hepatica gi:159461074; Sm, S. mansoni gi:256084837; Sj, S. japonicum gi:226487206.