Figure 1.
Touch impression of a rabies-negative domestic dog brain tested with the direct fluorescent antibody test (A) and direct rapid immunohistochemical test (B).
(A) No immunofluorescence observed in the brain processed by DFA. Magnification, ×400. (B) No magenta inclusions are visible on the blue neuronal background of the brain processed by dRIT. Magnification, ×200.
Figure 2.
Touch impression of a rabies-positive domestic dog brain tested with the direct fluorescent antibody test (A) and direct rapid immunohistochemical test (B).
(A) Apple-green immunofluorescent viral inclusions observed on the red neuronal tissue in the brain processed by DFA. Magnification, ×400. (B) Magenta viral inclusions are visible on the blue neuronal background of the brain processed by dRIT. Magnification, ×200.
Figure 3.
Phylogenetic representation of the genetic relationship between the rabies virus-positive sample (711/12) and representative canine and mongoose rabies virus variants circulating in southern Africa.
Table 1.
Summary of the viral RNA concentration and antigenic variants of the rabies-positive neuronal tissue samples that produced false-negative results subsequent to the application of the direct rapid immunohistochemical test using either one or both of the biotinylated monoclonal antibody preparations (MAb 1 or MAb 2).
Table 2.
Diagnostic sensitivity, specificity and Cohen's Kappa measure of agreement of the direct rapid immunohistochemical test using one of three biotinylated antibody preparations as well as the theoretical monoclonal antibody cocktail evaluated in this study.