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Figure 1.

Map of study area.

Iquitos is the largest city in the Peruvian Amazon (pop: 380,000), and is accessible only by boat or plane. There are approximately 500,000 people living in the study area shown on the right side of the map. Fluvial routes are the predominant mode of transportation in the region.

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Figure 2.

Ae. aegypti presence-absence by data source.

Results from pupal demographic surveys/ovitraps are shown for A) Historical data from MOH/NAMRU data in 2008, B) Historical data from MOH/NAMRU in 2011, and C) Data collected for this study in 2011-12 (N = 34).

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Table 1.

Entomological indices for communities positive for Ae. aegypti (collected data only).

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Figure 3.

Mann-Whitney Wilcoxon tests for median differences in Ae. aegypti positive vs. negative communities.

Significant differences (p<0.05) between Ae. aegypti positive vs. negative communities were detected in terms of human population size, distance from Iquitos, and the number of wet containers per house.

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Table 2.

Multivariable logistic regressions: Ae. aegypti risk factors at the community scale.

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Table 3.

Multivariable logistic regressions: Ae. aegypti risk factors at the house scale.

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Figure 4.

Larval and pupal productivity by container type.

A) Larval productivity, B) Pupal productivity.

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Table 4.

Proportion of positive containers by type.

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Table 5. Multivariable logistic regressions: Ae. aegypti risk factors at the container scale.

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Figure 5.

Geographic border of Ae. aegypti colonization along the Iquitos-Nauta highway.

The distance between the southernmost positive community, 5 de Abril, and the next community, San José, is approximately 6.49 km. The space between these two communities is characterized by forest cover with no human settlements (and therefore no oviposition sites). This forested area likely acts as a barrier to Ae. aegypti dispersal.

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