Figure 1.
Overview of cellular responses during an asymptomatic L. donovani infection.
Infected macrophages can produce TNF and IL-1β in response to L. donovani infection as part of the innate immune response. However, DC IL-12 production in response to L. donovani infection is required to drive the differentiation of antigen-specific CD4+ T cells into IFNγ- and TNF-producing Th1 cells. These cells can activate infected macrophages and monocytes to produce ROI and RNI that kill intracellular parasites. There are also reports in humans that Th17 and Th22 cells develop in asymptomatic, infected individuals, possibly driven by IL-23 and IL-6. However, the antiparasitic mechanism mediated by these CD4+ T cell subsets following L. donovani infection remains unknown. Although parasite-specific antibodies are readily detected in asymptomatic individuals, their role, if any, in control of infection and protection against reinfection is unknown. Abbreviations: MO, monocyte; Mφ, macrophage.
Figure 2.
Overview of cellular responses during a chronic L. donovani infection.
During an established L. donovani infection, a subset of regulatory DCs in the spleen can produce IL-10 that promotes the expansion of IL-10-producing regulatory T cells (Tr1), as well as inhibiting antimicrobial mechanisms in macrophages and other phagocytic cells (including suppression of ROI and RNI generation). IL-27 produced by regulatory DCs and macrophages, along with T cell–derived IL-21, can drive the differentiation of Th1 cells into Tr1 cells, as well as inhibit Th17 development. IL-10 produced by Tr1 cells can suppress antigen presentation, contributing to T cell dysfunction, as well as down-regulate CD4+ T cell IFNγ production. There has been a report that IL-10 can also be produced by Treg cells in the BM of VL patients. Although uptake of infected neutrophils undergoing apoptosis by macrophages contributes to the establishment of L. major infection in mice, no such mechanism has yet been described during L. donovani infection. Abbreviations: N, neutrophil.