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Figure 1.

Map showing federal states of Germany (a) and geographical origin of all bat specimens coming from Schleswig-Holstein (SH, N = 362); Bremen (HB, N = 4), Hamburg (HH, N = 10), Mecklenburg-Western Pomerania (MWP, N = 131), Lower Saxony (LS, N = 1252), Berlin (B, N = 484), Brandenburg (BRB, N = 644), Saxony-Anhalt (ST, N = 692), Saxony (SN, N = 247), North Rhine Westphalia (NRW, N = 76), Hesse (HE, N = 89), Thuringia (TH, N = 296), Rhineland-Palatinate (RP, N = 108), Saarland (SL, N = 53), Baden-Wuerttemberg (BW, N = 736), Bavaria (BY, N = 252) (b) and of E. serotinus (c) collected in the study described here, and of the bat rabies cases (dot (N = 46): E. serotinus, triangle (N = 3): M. daubentonii, square (N = 3): P. pipistrellus, P. nathusii and Pl. auritus (d).

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Table 1.

Number of bat samples per species investigated using FAT, RTCIT, RT-qPCR and RT-PCR.

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Figure 2.

Evolutionary relationships of EBLV-1 (a) and EBLV-2 strains (b) with a focus on 400 nucleotides long N-gene sequences (nt positions 1–400, numbering according to EF157976) derived from this study (boldface).

The Neighbor-Joining method (p-distance, 1000 pseudoreplicates) as implemented in MEGA 5 was used. Sequence number 998 LS represents the identical sequences 959, 5300, 5304, 7471, 7467, 11647, 15730, 16902, 16908, 18720, 21836, 24525, 24529, 24832, 25495, 31054.

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Figure 3.

Number of bat specimens tested (N = 3714, black) and rabies cases (N = 46, grey) per month during 1998 until June 2013.

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