Table 1.
Case definition for suspected cases of LF.
Figure 1.
Suspected cases of LF evaluated at the KGH Lassa Laboratory and numbers of patients admitted to the KGH Lassa Ward, 2008–12.
Non-admitted patients include those where only blood samples were submitted for screening from referral health-posts, patients dying en route to the hospital (DOA = dead on arrival), and patients not meeting the LF suspected case criteria (Table 1). Characteristics of study patients are compiled in Table S1.
Figure 2.
CFRs in suspected LF cases presenting to the KGH Lassa Ward by serostatus, 2008–12.
Panel A: CFR by serostatus. The presence of LASV Ag and anti-LASV IgM in serum of patients with verifiable outcomes was assessed by recombinant Ag− and IgM− capture ELISA, respectively. Panel B: Alternative calculation of CFRs. Ag+/IgM± plus Ag−/IgM+ compared to Ag−/IgM−. Statistical significance was determined using a logistic regression model predicting CFR (Table S3). NS = not significant.
Figure 3.
Anti-LASV IgG in suspected LF patients presenting to the Kenema Government Hospital, 2011–12.
Routine anti-LASV IgG serological testing was implemented at KGH in 2011. Panel A: Percentage of patients with anti-LASV IgG by serostatus. Panel B: Case fatality rates in patients with verifiable outcomes by LASV antigen, anti-LASV IgM, and anti-LASV IgG serostatus. Panel C: Case fatality rates in patients with verifiable outcomes by an alternative assessment of LF status (acute = Ag+/IgM±/IgG±, convalescent = Ag−/IgM+/IgG+, nonLF = Ag−/IgM+/IgG− or Ag−/IgM−/IgG± LASV antigen, anti-LASV IgM, and anti-LASV IgG serostatus. Logistic regression models predicting IgG-positivity and CFRs were used to carry out within and between group comparisons (Table S4). NS = not significant.
Figure 4.
Geographic distribution of patients presenting to the KGH with LASV antigenemia and anti-LASV IgM serpositivity, 2008–12.
Confirmed cases of LF as assessed by LASV Ag in serum or cases anti-LASV IgM are shown by year of presentation, district of residence and frequency of cases. Panel A: Patients presenting in 2008–9. Panel B: Patients presenting in 2010. Panel C: Patients presenting in 2011. Panel D: Patients presenting in 2012.
Figure 5.
Monthly distribution of suspected LF cases presenting to the KGH Lassa Ward by serostatus, 2008–2012.
Panel A: antigenemic Lassa fever cases (Ag+/IgM±). Panel B: Patients with serum anti-LASV IgM (Ag−/IgM+). Panel C: Patients with no Lassa virus seropositivity (Ag−/IgM−). The monthly frequency distributions differed between each of the serostatus group comparisons as assessed using a Poisson regression model (p<.001 for all serostatus comparisons; data not shown).
Figure 6.
Age distribution of cases presenting to the KGH Lassa Ward, 2008–12.
Panel A: Age distributions of patients presenting while antigenemic (Ag+/IgM±). Panel B: Age distributions of nonantigenemic patients presenting with serum anti-LASV IgM (Ag−/IgM+). Panel C: Age distributions of nonantigenemic patients presenting without anti-LASV IgM seropositivity (Ag−/IgM−). In Panels A–C yellow portion of bars represent patients who were discharged and black portion of bars represent patients who died. Panel D: Age demographic for the population of Sierra Leone (2010 estimate). Among patients who died, the age distributions differed significantly between the Ag+/IgM± and Ag−/IgM− groups (p = .005). Distributional comparisons were carried out using the Kolmogorov-Smirnov technique (Table S5).
Figure 7.
Gender and self-reported pregnancy status of suspected Lassa fever cases presenting to the KGH Lassa Ward, 2008–2012.
Panel A: Frequency of suspected Lassa fever cases by gender and serostatus. Panel B: Cases fatality rates by gender and serostatus. Panel C: Percentage of female patients of childbearing age with self-reported pregnancy status by serostatus. Panel D: Case fatality rates in female patients with self-reported pregnancy status Pregnancies are self-reported and therefore likely underestimated as pregnancy tests were not routinely available. Logistic regression was used for group comparisons (Tables S6 and S7). NS = not significant.
Figure 8.
Case fatality rates for suspected LF cases by ribavirin treatment status and serostatus.
The presence of LASV Ag in serum of patients with observed survival outcomes and verified treatment status was assessed by recombinant Ag− and IgM-capture ELISA. Statistical significance for within and between group comparisons was determined using a multivariate logistic regression model (Table S9). NS = not significant.