Figure 1.
Protein profile of the A. suum ES products.
Protein profile of the A. suum L3-egg, L3-lung and L4 ES products displayed on a 12% SDS-PAGE stained with Coomassie blue. Each lane was loaded with 15 µg of protein. Molecular weight markers are indicated to the left. The 10 gel slices used in the trypsin digests are indicated on the right.
Figure 2.
Venn diagram of similar proteins.
Venn diagram showing the distribution of the number of proteins identified in ES products from L3-egg, L3-lung and L4 of A. suum. The proteins identified are listed on the right.
Table 1.
Protein identifications in A. suum L3-egg ES products.
Table 2.
Protein identifications in A. suum L3-lung ES products.
Table 3.
Protein identifications in A. suum L4 ES products.
Figure 3.
Gene Ontology terms relating to molecular function assigned to the proteins identified in ES products from L3-egg, L3-lung and L4 of A. suum.
Table 4.
List of glycosyl hydrolases identified in the A. suum genome, their sequence length and their gene levels in different larval stages.
Figure 4.
Phylogenetic tree and signature motifs of GH31 proteins.
A. Unrooted phylogenetic tree of the A. suum GH31 proteins (with a minimum sequence length of 700 amino acids) and other selected eukaryotic GH31 protein following neighbour-joining analysis. The values at the branch nodes represent bootstrap values (maximum 1000). B. Comparative analysis of the amino acids around the catalytic nucleophile (Trp and Asp) of GH31 proteins for the C. elegans GH31 proteins AAGR1-4 and the A. suum GH31 proteins (GS_04731, GS_05082, GS_06701, GS_08447, GS_13054, GS_17123, GS_17323, GS_18807, GS_19777, GS_20796, GS_21706, GS_22047 and GS_23879).
Figure 5.
qPCR and glucosidase hydrolytic activity analysis of GH31 proteins.
A. A qPCR analysis for two GH31 proteins on cDNA produced from different larval stages and adult worm tissues. B. Comparison of the glucosidase hydrolytic activity in water-soluble (white bars) and water-insoluble extract (black bars) from different adult tissues. Results are shown as average + SD. Substrates used in the assays were lactose, maltose and sucrose. (Int F: female intestine; Int M: male intestine; Rep F: female reproductive system; Rep M: male reproductive system; Cut F: female cuticle; Cut M: male cuticle). (* P<0.05).