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Figure 1.

Geographical locations of confirmed RABV cases in Trinidad during the period 1997 to 2010.

Locations of cases are indicated by circles, with black circles indicating locations from which RABV sequences were derived for the current study and white circles designating unsampled locations.. For sampled locations, the number of cases from which sequences were derived and their date and species of origin are indicated in blue. The location of co-circulation of Trinidad I and Trinidad IIa lineages is also indicated by an asterisk.

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Table 1.

Trinidadian sequences used for phylogenetic analysis identified by geographical, temporal and species origin.

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Figure 2.

Bayesian Maximum Clade Credibility (MCC) tree inferred for RABV in the Americas based on N gene sequences (363 nt) from South and Central America [including Mexico] and Trinidad.

Clade credibilities of 95% and over are indicated in black at the relevant nodes. Terminal branches are coloured according to the sampled location and internal branches are coloured according to the most probable (modal) location of their parental nodes. Major clades and Trinidadian lineages are labeled accordingly. Chiropteran phylogenetic clusters are identified by dotted bar lines to the right and labeled according to group (species) designation which is further described in the inset.

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Figure 3.

(A) Enlargement of the section of MCC tree containing Trinidadian lineages.

The location state probabilities for selected nodes are shown as percentages. The estimated dates of divergence from mainland lineages and mean dates of existence for the most recent common ancestor (MRCA) for lineages containing Trinidadian sequences (with 95% HPD in parentheses) are shown in blue and black respectively to the left of relevant nodes. (B) Histogram inserts indicating the location state probabilities for the estimated introductions of RABV that gave rise to Trinidad lineages I (I1), IIa (I2) and IIb (I3).

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