Figure 1.
Proportion of Ae. aegypti with a detectable infection after being held at low temperatures.
Females were held at 16°C, 20°C and 26°C constant, and 20°C with a large DTR and sampled at days 7, 14, 21 and 28 post-exposure to an infectious DENV-1 blood meal. A) Body infection, representing a detectable infection of the midgut tissue. B) Levels of infection in head tissue, representing a detectable disseminated infection.
Table 1.
Temperature effects on dissemination probability of Ae. aegypti infected with DENV-1 exposed to low temperatures.
Figure 2.
Proportion of Ae. aegypti with a detectable infection after being held at high temperatures.
Females were held at 30°C and 35°C constant, and 30°C with a small DTR and sampled at days 3, 6 and 9 post-exposure to an infectious DENV-1 blood meal. A) Body infection, representing a detectable infection of the midgut tissue. B) Levels of infection in head tissue, representing a detectable disseminated infection.
Figure 3.
Survival of females exposed to DENV-1 from various constant and cyclic temperature regimes.
A) Females held at low temperatures and a 26°C control. Despite the overall effect of temperature (p<0.0001), there were no differences in the survival curves after 28 days, between any of the three low temperature treatments (p>0.7). At 26°C constant, mortality was greater than in each of the low temperature treatments (p<0.001). B) Females held at high temperatures. Temperature influenced overall survival curves (p = 0.006), but only the curves of 30°C plus small DTR and 35°C constant were statistically different from each other (p = 0.001).