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Figure 1.

Number of schools and stool samples for assessing infection intensity of STHs in Jimma, Ethiopia.

aEligibility criterion for the schools was hosting grade 1–8 students (age from 5–18 years); bExcluded from analysis since <60 samples were provided.

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Figure 2.

Participation and compliance in assessing drug efficacy against STHs in schoolchildren in Jimma, Ethiopia.

At follow-up, pooling of 10, 20, or 60 samples was not always possible: * pool10 includes: pools of 9 (n = 12) and pools of 10 (n = 36) samples; ** pool20 includes: pools of 18 (n = 5), pools of 19 (n = 2) and pools of 20 (n = 17) samples; and *** pool60 includes: pools of 55 (n = 1), 56 (n = 1), 58 (n = 1) and 59 (n = 1) and pools of 60 (n = 4) samples.

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Figure 3.

Procedure to obtain pools of 10, 20, and 60 individual stool samples.

Sixty individual samples were arranged in 6 rows with each row consisting of 10 individual samples, subsequently 6 pools of 10, 3 pools of 20, and 1 pool of 60 individual samples, resulting in total of 10 pooled samples per school.

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Figure 4.

Agreement in FEC of STHs between individual and pooled samples.

Each of the 9 scatterplots represents the agreement in mean individual FEC and pooled FEC of stool samples. The plots in column A, B and C represent the A. lumbricoides, T. trichiura, and hookworm, respectively. The plots in top, middle and bottom row represent pool sizes of 10, 20 and 60, respectively. The magnitude of correlation for each plot is based on the Spearman correlation coefficient (Rs).

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Table 1.

Mean fecal egg counts for STHs based on individual and pooled samples.

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Table 2.

Fecal egg count reduction of mebendazole for STHs assessed by pooled and individual samples.

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