Figure 1.
Survival curves for four experimental groups of Ae. aegypti females (n = 50), based on the daily probability values computed by the Kaplan-Meier model for Bioassay 1.
Mosquitoes were challenged with either the DENV-2 (1×107 PFU/mL) (SV = black circle, CI = black triangle) and/or exposed for 24 hours to 1.6×108 conidia mL−1 of M. anisopliae (SF = white triangle); controls (white circle) were exposed to neither treatment.
Figure 2.
Viral infection rate in midgut and head.
Number of Ae. aegypti (50 per treatment) virus-free females (white square), with the DENV-2 virus in midgut (grey square) and in both midgut and head (black square) that died at different days until the day 7 PI when survivors were sacrificed and tested for viral presence. 2a: Mortality for SV mosquitoes. 2b: Mortality for CI females.
Figure 3.
Effect of the fungus on reproduction.
Impact of infections with M. anisopliae and/or the DENV-2 on the number of eggs per female (bars), and duration of the gonotrophic cycle (GC) in days (black circles) of Ae. aegypti females after exposure to CI, SF, SV treatment plus control (three replicates, 20 females each per treatment). CI and SF were not different significantly, but both were lower than SV and Control.
Figure 4.
Daily survival probability (p) for Ae. aegypti females in both SV (white circle) and CI (black circle) treatment computed by regression of number of females died daily [ln (X+1)] on the day PI.