Table 1.
Samples used to assess sensitivity and specificity of Wb123 LIPS.
Figure 1.
Sequence alignment of Wb123 with homologous proteins in Brugia malayi (Bm123), Loa loa (Ll123), and Onchocerca volvulus (Ov123). Boxed blue amino acids are those that show 100% conservation with those of Wb123.
Figure 2.
ROC Analysis Wb123 antibody responses.
Receiver operating characteristic (ROC) analysis using serum samples from Wuchereria bancrofti-infected subjects (n = 43) and normal North American non-travelers (n = 50) for IgG (Left panel) and IgG4 (Right panel) anti-Wb123 antibodies.
Figure 3.
Wb123-specific IgG and IgG4 in W. bancrofti and related helminth infections.
IgG and IgG4 antibodies to Wb123 distinguish Wuchereria bancrofti-infected subjects from those with related filarial and non-filarial helminth infection. IgG (Left panel) or IgG4 (Right panel) antibodies to Wb123 in individual serum samples from those with Wuchereria bancrofti (Wb), Onchocerca volvulus (Ov), Loa loa (Ll), Mansonella perstans (Mp), other helminth infections, or no infection (Normal). Each dot represents an individual sample and the horizontal line is the geometric mean (GM). The dashed line represents the cutoff between negative and positive based on ROC analysis.
Table 2.
Sensitivity and specificity of Wb123 IgG and IgG4 LIPS.
Figure 4.
Performance of Wb123-specific IgG LIPS assay in blinded analysis.
Blinded analysis of IgG anti-Wb123 antibodies from samples obtained from Wuchereria bancrofti-infected patients from Haiti (Haiti MF pos), those from a Wb non-endemic region of Haiti (Haiti non-exposed), US non-travelers, those with other non-filarial parasitic infection, or those with Brugia malayi infection. Each dot represents an individual subject and the horizontal line is the GM. The dashed line represents the cutoff between negative and positive based on ROC analysis.
Figure 5.
Antibodies to Wb123 fail to normalize following definitive anti-filarial treatment.
IgG (Top panel) and IgG4 (Middle panel) anti-Wb123 antibodies in two individual patients followed longitudinally over an 8 (red line) or 17 (blue line) year period following definitive anti-filarial chemotherapy. Circulating antigen levels in the same two subjects is shown (Bottom panel). Gray shaded boxes show the normal range for each assay.