Figure 1.
Skin lesion caused by Leishmania tropica in female of CcS-16 strain at week 43 after infection.
Figure 2.
Kinetics of skin lesion development of CcS/Dem strains after infection with L. tropica.
Individual strains are marked with different colors. The columns show median values of lesion size in females (A) and males (B). Figure summarizes data from two independent experiments.
Table 1.
Sex differences in lesion size (week 8) after L. major infection.
Figure 3.
Number of parasites cultivated from lymph nodes of mouse strains 21 and 43 weeks after infection.
Mice were killed at week 21 (A) and week 43 (B) after infection. Asterisks show strains that exhibited parasite load significantly different from BALB/c. Brackets indicate strains with differences between males and females. Data are presented as mean ± SD.
Figure 4.
Leishmania tropica parasites inside the macrophage.
A smear of the inguinal lymph node of BALB/c male was stained with the anti-Leishmania lipophosphoglycan monoclonal antibody (CLP003A, Cedarlane, Hornby, Canada) and TRITC labeled IgM (115-025-020, Jackson Immunoresearch, West Grove, PA, United States of America), all diluted 1∶100. Nuclei of the cells were stained with DAPI. Parasites are shown with arrows.
Figure 5.
Parasites in hematoxylin-eosin stained spleen smears.
Arrows show a group of parasites among spleen cells in infected BALB/c male (A); noninfected control BALB/c male without parasites (B).
Figure 6.
Relationship between chemokine expression and lesion size development during the course of L. tropica infection.
Kinetics of skin lesion development (median, left y axis) and serum levels of CCL2, CCL3 and CCL5 (median values; right y axis) in females are shown. Figure summarizes data from two independent experiments (21 and 43 weeks of infection).
Figure 7.
Comparison of kinetics of serum level of CCL3 and CCL5 in females and males of strains CcS-16 and BALB/c.
Kinetics of CCL3 levels (median values) in serum of BALB/c (A) and CcS-16 (B) and serum levels of CCL5 (median values) in BALB/c (C) and CcS-16 (D) mice is shown. Figure summarizes data from two independent experiments (21 and 43 weeks of infection).