Figure 1.
Sampling sites of wild populations of Triatoma infestans in Bolivia.
The sites were numbered from 1 to 36, Bolivian department names are indicated, for the DTU T. cruzi results see in Table 1.
Table 1.
Geographical origin, ecotopes of wild triatomines and T. cruzi identification by MMPCR.
Figure 2.
Illustrating electrophoresis of MMPCR products.
Ez-vision stained 3% agarose gel containing MMPCR products obtained from DNA extracts of reference strains and current digestive tracts of T. infestans. Lane 1, sample Tor05; lane 2, PCR negative sample; lane 3, the molecular weight marker Smart Ladder (Eurogentec, Angers, France); lane 4–6, reference strains (M6241cl6, P209cl1 and MNcl2 respectively); lane 7–8, sample Char09 (two independent PCR); lane 9, sample Lur112. See Table 2 for DTUs information.
Table 2.
Variable sites of glucose phosphate isomerase gene of T. cruzi identified in wild T. infestans compared with reference stocks.