Figure 1.
IDs at different stages of their development.
The IDs of optimal size (A, B), underdeveloped (C) and overdeveloped IDs (D) are shown. The location of the IDs under the cuticle in thoracic segments of 4th instar larvae (A, C, D) and dissected IDs (B) are indicated by arrows.
Figure 2.
The dynamics of mitosis in IDs and brain ganglia.
The mitosis in IDs of two different sizes (A), in IDs at two different temperatures (B) and in one ID and two brain ganglia (C) are compared.
Figure 3.
Chromosomes from IDs at different stages of mitosis.
Prophase (A-C), prometaphase (D), metaphase (E), and anaphase (F) are shown. Chromosomes 1, 2, and 3 are indicated by numbers.
Table 1.
Comparison of the Aedes aegypti chromosomes from different sources.
Figure 4.
Development of idiograms for the ID chromosomes.
Initial color images of YOYO-1 stained chromosomes (A), negatives of the same images converted to grey scale (B) and idiograms with BAC clone locations (C) are shown. Chromosomes 1, 2, and 3 are indicated by numbers, p – the short arm, q- the long arm of the chromosome. Chromosomal landmarks are shown by stars. The positions of the BAC clones contained specific genetic markers (AeW, AEGI8, LF103, etc.), and 18S rDNA are indicated by red bars.
Figure 5.
Examples of in situ hybridization of DNA probes to ID chromosomes.
18S rDNA probe and 10 BAC clones were mapped to the chromosomes 1 (A, B); 2 (C, D); and 3 (E, F). Chromosomes are indicated by numbers, p – the short arm, q- the long arm of the chromosome. The positions of the signals on the chromosomes are indicated by arrows. The BAC clones are named by genetic markers which they are carrying (Table 2).
Table 2.List.
of the probes hybridized to the chromosomes from imaginal discs of Aedes aegypti.