Figure 1.
Full length amino acid sequence of scabies mite peritrophin 1 (SsPTP1) aligned with other arthropod peritrophins.
The scabies mite peritrophin SsPTP1 is shown in bold in a sequence alignment with Anopheles gambiae peritrophin 1 (AF030431), peritrophin from Aedes agypti (AAL05409) and a peritrophin matrix protein from Tribolium castaneum (XP_974131). The following predictions are shown: signal sequences (underlined); chitin binding domains (grey background); N-glycosylated residues (red box); O-glycosylated residues (yellow background). Cysteine residues predicted to form disulfide bonds within the chitin binding domains are shown in magenta. A blue and a red bar indicate the sequence of the chitin binding domain CBD1 and the peptide c2, respectively.
Figure 2.
Specificity of mouse antisera raised against a recombinant chitin binding domain of SsPTP1.
Shown is a series of purified scabies mite proteins on a coomassie-stained SDS-PAGE (A) and a corresponding western with a peritrophin-specific antibody (B). The gel (A) demonstrates the purity of the recombinant peptide CBD1 (lane 2) and the fusion protein TSP-CBD1 (lane 3) and (B) the specificity of the antibody raised against CBD1, confirming no cross-reaction with unrelated scabies mite proteins SMSB3a (lane 1), SMIPP-S D1 (lane 5), SMIPP-S I1 (lane 6) and BSA (lane 4).
Figure 3.
Binding of recombinant peritrophin fusion protein TSP-CBD1 by affinity chromatography to chitin magnetic beads.
(A) Shown are elution profiles of the peritrophin fusion protein TSP-CBD1, Schistosoma mansoni TSP protein and bovine serum albumin (BSA) (negative controls), and wheat germ agglutinin (WGA; positive control). Data are means of n = 4 independent experiments measured in duplicate. 100 µg protein was loaded in each chromatography run. E1, elution (8M urea); E2, elution (5% SDS); E3, elution (10% SDS); matrix-bound, the amount of protein estimated to be still bound to the matrix after E1-E3 elution; n.d., not detectable (B) Western blotting of chitin binding chromatography fractions from an representative TSP-CBD1 run. Immunodetection was performed using polyclonal mouse antibodies against peritrophin peptide CBD1. Lanes E1-E3 show elution fractions and lane M protein bound to chitin matrix beads.
Figure 4.
Immunohistological localisation of SsPTP1 in scabies mite gut and excreted mite feces.
Histological sections were probed with antibodies raised against the peritrophin peptide CBD1 (A2, B2), against peptide c2 (A6, B6), with anti-human IgG (A3, A7, B3, B7) and corresponding pre-immune sera (A4, A8, B4, B8). Red staining indicates binding of primary antibody. A schematic diagram in the left panel (A1 and A5; B1 and B5) outlines the features in the histological sections. The mite gut and fecal pellets are shown in red, the mite body in gray, the burrow in white and the epidermis in blue. b, burrow; f, feces; g, gut; m, mite. Scale bars (100 µM) indicate the magnification level.
Figure 5.
Deglycosylation of native scabies mite peritrophin 1 and binding to the human complement factor MBL.
Scabies mite homogenates were incubated for 24 hours (upper panel) or 3 days (bottom panel) in either buffer alone (lane 1), or with PNGase F (lane 2), four O-glycosidases (lane 3), or PNGase F and four O-glycosidases (lane 4) at 37°C. The preparations were separated by SDS-PAGE in comparison with a sample of fresh mite homogenate (lane 5). Western blots were probed for SsPTP1 using anti-CBD1 sera (A), or for MBL using an anti-MBL antibody after incubation of the blot with 50% normal human serum (B) or with buffer without serum (C). In blot (C) an additional lane was loaded with 1% normal human serum in PBS (lane S). Arrows point to a single band corresponding to the 32kDa subunits of human MBL. The experiment was repeated three times.
Figure 6.
Immunohistological localisation detects complement factor C9, but not the activated SC5b-9 complex in scabies mite infested human skin sections.
Histological sections were probed with antibodies raised against human SC5b-9 neoantigen (2) and against complement factor C9 (6), with anti-human IgG (3 and 7) and the respective pre-immune sera from goat (4) and rabbit (8). Red staining indicates binding of primary antibody. A schematic diagram in the left panel (1 and 5) outlines the features in the histological sections. The mite gut and fecal pellets are shown in red, the mite body in gray, the burrow in white and the epidermis in blue. b, burrow; f, feces; g, gut; m, mite. Scale bars (100 µM) indicate the magnification level.