Table 1.
The characteristics of the study populationa.
Figure 1.
Magnitude of DENV viremia by illness day.
Levels of DENV-1, -2, -3 and -4 genome equivalent cDNA copies per millilitre were determined in serial plasma samples from patients with A) primary DF (n = 26), B) secondary DF (n = 144) and C) secondary DHF (n = 64). Data are median and IQR. *** P<0.001 ** 0.001<P<0.01 * 0.01<P<0.05 t 0.05<P<0.1. Data from patients with DENV-1 and -2 primary DHF (n = 3 and n = 1 respectively) and DENV-4 secondary DHF (n = 1) are not displayed because the number of these patients was too small for statistical analysis. The numbers below the graph indicate the numbers of patients at each time point. The dashed line represents the assay limit of detection.
Figure 2.
Magnitude of DENV-1 viremia by illness day.
Levels of DENV-1 genome equivalent cDNA copies per millilitre were determined in serial plasma samples from DENV-1 infected patients with primary DF (n = 15), primary DHF (n = 3), secondary DF (n = 91) and secondary DHF (n = 33). Data are median and IQR. *** P<0.001 ** 0.001<P<0.01 * 0.01<P<0.05 t 0.05<P<0.1. The numbers below the graph indicate the numbers of patients at each time point (2 per patient for most of the time points). The dashed line represents the assay limit of detection.
Figure 3.
Time to resolution of DENV viremia.
Kaplan-Meyer survival analysis of time to resolution of plasma viremia in all viremic A) or DENV-1 only B) patients.
Figure 4.
Time to resolution of NS1 antigenemia.
Kaplan-Meyer survival analysis of time to resolution of NS1 antigenemia in all viremic A) or DENV-1 only B) patients. Two patients NS1 negative at enrolment were later positive and for the purposes of analysis were considered positive at the time of enrolment.
Figure 5.
Kaplan-Meyer survival analysis of fever clearance time in all viremic patients. Two patients afebrile at enrolment developed fever soon after and for the purposes of analysis were considered febrile at the time of enrolment.
Figure 6.
Profile of haematological parameters and viremia in DENV-1 infected patients.
Medians of viremia levels, WBC and platelet counts, hemoconcentration, and percentages of lymphocytes and neutrophils were determined in serial plasma samples from DENV-1 infected patients with A) primary DF, B) secondary DF, C) primary DHF and D) secondary DHF. The numbers below the graph indicate the numbers of patients at each time point. For reasons of clarity, error bars are not shown.