Table 1.
Baseline characteristics of included patients.
Figure 1.
Histopathological features of myocardial samples.
Slides of hematoxilin-eosin- (left column) and picrosirius red- (right column) stained myocardial sections of representative patients with CCC, IDC and IC and individuals without cardiomyopathies (N). Myocardial hypertrophy characterized by fiber and nuclear enlargement is evident in the CCC, IDC and IC groups. Lymphocytic myocarditis is present only in the CCC group. Interstitial fibrosis stained red in picrosirius stain is present in the CCC, IDC and IC groups.
Figure 2.
Analysis of differential protein expression of energy metabolism enzymes by immunoblotting.
(A) ATPα: ATP synthase alpha subunit, (B) ATPβ: ATP synthase beta subunit, (C) CKM: creatine kinase M and (D) CKMit: mitochondrial creatine kinase. The densitometric values of each protein for each sample were normalized by the values of GAPDH. The horizontal lines show statistically significant changes: *p<0.05; **p<0.01; ***p<0.001.
Figure 3.
mRNA expression of energy metabolism enzymes by real time RT-PCR.
(A) ATPα: ATP synthase alpha subunit, (B) ATPβ: ATP synthase beta subunit, (C) CKM: creatine kinase M and (D) CKMit: mitochondrial creatine kinase. The horizontal lines show statistically significant changes: *p<0.05.
Figure 4.
Analysis of creatine kinase enzymatic activity.
The values were normalized by the amount of protein from each sample. The horizontal lines show statistically significant changes: *p<0.05; **p<0.01.