Table 1.
Human serum panel in Vietnamese study site (n = 298).
Figure 1.
Representative sensorgrams for binding of monoclonal antibody Ab3 and DENV-1 NS1.
Increasing concentrations of dengue-1 NS1 were sequentially injected onto 70 RU of Ab3 captured by anti-mouse IgG. The reference-subtracted data (black curve) were fitted using 1∶1 binding algorithm (red curve).
Table 2.
Binding kinetics of anti-NS1 MAbs to dengue NS1 antigens determined by 1∶1 binding model.
Figure 2.
Analytical sensitivity of Dengue Early Rapid test for recombinant dengue NS1.
The test line signal intensity was measured against a serial two-fold dilution series of DENV-1 NS1 (A), DENV-2 NS1 (B), DENV-3 NS1 (C) and DENV-4 NS1 (D) from 512 ng/mL to 0.5 ng/mL. The analytical limit of detection was determined as the lowest concentration required to produce a positive result of 8.5 mABS (absorbance) units using interpolation from a non-linear regression. An absorbance value of 8.5 mABS was determined to be the cut-off value for visualisation of the test line.
Table 3.
Accuracy of Dengue Early Rapid (NS1) test in Vietnamese study site.
Table 4.
Human serum panel from Malaysian study site (n = 293).
Figure 3.
Accuracy of dengue rapid tests at the Malaysian study site.
Diagnosis was confirmed using a combination of HI, virus isolation, RT-PCR and IgM ELISA (n = 293). Sensitivity (%) between groups was compared using Fisher's exact test.
Figure 4.
Clinical sensitivity of rapid tests for acute dengue over the course of illness.
Data was obtained from laboratory-confirmed cases of dengue at the Malaysian study site (n = 263).