Figure 1.
Immunoreactivity of the α-E/S antibody is primarily specific for adult S. ratti E/S.
α-E/S IgG recognizes primarily Strongyloides E/S antigens (A): Western blot analysis of (E/S) adult worm E/S, (AWAg) adult worm antigen, (L3Ag) 3rd stage larval antigen and (RGAg) soluble naïve rat gut antigen, using α-E/S IgG at a dilution of 5.5 µg/ml. A single immunodominant ∼75 kDa cross-reacting band was observed in the uninfected rat faecal supernatant immunoprecipitation experiment. Other immunoreactive protein bands are recognised by the α-E/S Ab and are not seen in uninfected rat gut extract. These S. ratti-specific proteins were detected at approximate molecular weights of 38 kDa, 40 kDa, 60 kDa and 70 kDa in AWAg, with additional 35 kDa, 50 kDa, 65 kDa, 100 kDa and >100 kDa bands being observed in E/S. (B) Immunoperoxidase-stained parasitic adult worm sections, probed with either (upper panel) 1∶500 preimmune rabbit serum, or (lower panel) α-ES IgG diluted 5.5 µg/ml in PBS. Scale bar = 20 µm. Legend: I = intestine; Ov = ovaries; C = cuticle. Arrows highlight the specific ovarian and intestinal cells recognised by this antibody.
Figure 2.
Formalin preservation allows specific discrimination of infected rat fecal supernatant from uninfected fecal supernatant.
(A) Effect of assay specificity after various treatments. Pooled uninfected fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant collected from 2 rats infected with S. ratti (13 and 21 dpi) (iRFS) were extracted simultaneously in PBS-T and 4% formalin (4%F). Only extraction in formalin led to a positive discrimination between nRFS and iRFS. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Detection of known amounts of E/S products diluted in PBS or uninfected rat fecal supernatant (nRFS) extracted in 4% formalin. The lowest concentration of E/S products detected was 80 ng/ml and 325 ng/ml when diluted in PBS and nRFS, respectively. All points are mean ± SEM of OD values obtained from 4 samples.
Figure 3.
No cross reactivity is observed with purified E/S antigens or faecal supernatants collected from various helminths.
(A) Testing of the Strongyloides coproELISA with E/S products collected from other helminths. All Ancylostoma and Schistosoma E/S products tested negative even at the high concentration of 100 µg/ml. Pooled uninfected rat fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant (iRFS) collected from two rats (13 and 21 days post infection). iRFS and 1.3 µg/ml S.ratti E/S (+) tested positive. All points are mean ± SEM of OD values obtained from 4 identical samples. (B) Specificity for Strongyloides coproantigen detection. White bars and black bars represent data from two different experiments. Diluted samples were prepared from undiluted fecal supernatant to 1∶4 by doubling dilution in PBS. Uninfected mouse fecal supernatant (nMFS), Trichuris muris and Schistosoma mansoni supernatants test negative even when applied undiluted. Uninfected rat fecal supernatant (nRFS) and infected rat fecal supernatant (iRFS) tests negative and positive, respectivly. All points are mean ± SEM of OD values obtained from duplicate samples. Assay cuttoff for negative reading is represented by the grey box.
Figure 4.
Effect of sample storage on rat fecal supernatant and detection of heterologous S. stercoralis coproantigen.
(A) Pooled uninfected rat fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant (iRFS) collected from two rat (13 and 21 dpi) had previously been stored at −20°C and consistently tested negative and positive, respectively. Numbered samples 123, 150, 151, 160 and 161 represent samples collected from rats infected with known amounts of S. ratti infective larvae. Extracted fecal supernatant stored at 4°C displayed erratic absorbance values, as fecal supernatants from both uninfected and infected rats test positive. All unprocessed S. ratti infected feces stored in formalin (4% or 10%) tested positive for coproantigen when extracted after storage. Uninfected rat fecal feces tested negative when stored unextracted. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Uninfected human fecal supernatant (nHFS) and Strongyloides-infected human fecal supernatants collected daily from a single patient with clinically diagnosed strongyloidiasis (SS01-SS03) were extracted simultaneously with 4% formalin and 10% formalin. All points are mean ± SEM. Assay cuttoff for negative reading is represented by the grey box.