Figure 1.
Algorithm for molecular characterization of T. cruzi DTUs based on five molecular markers.
1 Souto et al., 1996, 2 Liarte et al., 2009, 3 Brisse et al., 2000, 4 Duffy et al., 2009 and 5 Falla et al., 2009.
Figure 2.
Amplification products of the DNAst, intergenic region of mini-exon gene and 24Sα rDNA region performed.
Figure 2A. Amplification product in 2% agarose gels of DNAst. Lane 1-6 Patients 111- 116 TcII-TcVI, Lane 7. Patient 87, Lane 8. 50-bp Weight Marker. Figure 2B. Amplification product in 2% agarose gels of the D7 24Sα rDNA region. Lane 9. 100-bp Weight Marker, Lane 10. Patient 87 TcI, Lane 11. Patient 98 TcII-TcVI, Lane 12–13. Patients 101-107 TcI. Figure 2C. Amplification product in 2% agarose gels of the intergenic región of mini-exon gene. Lane 1. 100-bp Weight Marker, Lane 2. Patient 12 TcII-TcVI, Lane 3. Patient 34 TcII-TcVI, Lane 4–8. Patients 33-34-35-36 TcI.
Figure 3.
Amplification products of the 18S, 24Sα rDNA and qPCR DNAst Tm peaks for the detection of TcIII and TcIV DTUs.
Figure 3A. Amplification products in 2% agarose gels of D7 24Sα rDNA region in T. cruzi. Lane 1. Negative control, Lane 2. Patient 54 TcIV, Lane 3. Patient 76 TcIII, Lane 4. Patient 112 TcIII, Lane 5. Patient 212 TcII, Lane 6. Patient 12 TcII, Lane 7. Negative control, Lane 8. 50-bp Weight Marker. Figure 3B. Amplification products in 2% agarose gels of 18S rDNA region in T. cruzi. Lane 1. Patient 57 TcIV, Lane 2. Patient 54 TcIV, Lane 3. Patient 76 TcIII, Lane 4. Patient 112 TcIII, Lane 5. Patient 212 TcII, Lane 6. Patient 12 TcII, Lane 7. Negative control, Lane 8. 50-bp Weight Marker. Figure 3C. Melting temperature peaks of TcIII and TcIV samples by qPCR real-time.
Figure 4.
Prevalence of electrocardiographic, radiologic and echocardiogram alterations in chronic Chagasic patients from Colombia.
(A) Prevalence of alterations based on 240 chronic Chagasic patients, (B) Prevalence of alterations based on 20 selected chronic Chagasic patients. Right bundle-branch block (1), left bundle-branch block (2), left anterior fascicular block (3), left posterior fascicular block (4), ventricular premature beats (5), first degree atrioventricular block (6), Mobitz type I atrioventricular block (7), sinus bradycardia (8), primary ST-T changes (9), abnormal Q-waves (10), low voltage QRS (11), atrial fibrillation (12), Mobitz type II atrioventricular block (13), complete atrioventricular block (14), complex ventricular arrhythmias (15), evidence of regional wall motion abnormality (16), reduced global left ventricular function and increased cardiothoracic ratio (17). (*) Statistically significant comparisons of TcI vs. TcII cardiac alteration using Tukey test.