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Figure 1.

Trial profile.

*All L.loa positive patients were additionally assigned to this group (numbers and dropouts provided in italics).

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Table 1.

Characteristics of patient groups that completed the trial (enrolment, treatment allocations and all follow ups).

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Table 2.

incidence and severity of adverse events during doxycycline and following ivermectin treatment.

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Table 2 Expand

Figure 2.

Changes in Onchocerca volvulus microfilaridermia from baseline at 4 months, 12 months and 21 months post treatment with doxycycline + ivermectin, doxycycline only or ivermectin only.

Arrows indicate commencement of intervention (black arrow; doxycycline, red arrow; ivermectin).

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Figure 2 Expand

Table 3.

Changes in O. volvulus microfilaridermia at 4, 12 and 21 months after the start of treatment.

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Table 3 Expand

Figure 3.

Immunohistochemical assessment of Wolbachia and nematode viability within adult O. volvulus tissue derived from trial patients at 21 months after the start of treatment.

A: WSP-specific immunostaining (arrow) of Wolbachia in O. volvulus adult female after ivermectin only treatment, B: Lack of WSP staining in O. volvulus from a doxycycline + ivermectin treated individual. C: APR staining (arrow) of viable adult female O. volvulus after ivermectin only treatment. D: Lack of APR staining in a non-viable adult worm from a doxycycline treated individual.

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Table 4.

Changes in Wolbachia surface protein expression and wsp DNA copy number in onchocercomas 21 months following start of treatment.

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Table 5.

Histological analysis of embryonic stages within uteri and released mf in onchocercoma tissue.

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Table 6.

Analysis of USG detection of adult O. volvulus worm movement 21 months after the start of treatment.

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Table 7.

O. volvulus viability 21 months after the start of treatment.

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Figure 4.

Changes in Loa loa microfilaridermia from baseline at 4 months, 12 months and 21 months post treatment with doxycycline + ivermectin, doxycycline only or ivermectin only.

Arrows indicate commencement of intervention (black arrow; doxycycline, red arrow; ivermectin).

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Figure 4 Expand

Table 8.

Changes in L. loa parasitaemia at 4, 12 and 21 months after the start of treatment.

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Figure 5.

Changes in Mansonella perstans microfilaridermia from baseline at 4 months, 12 months and 21 months post treatment with doxycycline + ivermectin, doxycycline only or ivermectin only.

Arrows indicate commencement of intervention (black arrow; doxycycline, red arrow; ivermectin).

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Figure 5 Expand

Table 9.

Changes in M. perstans parasitaemia at 4, 12 and 21 months after the start of treatment.

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