Table 1.
Parasite culture media.
Table 2.
Amaxa nucleofection assays.
Table 3.
In vitro cycle timing and virulence of various T. congolense strains.
Figure 1.
Inducible expression of EGFP through the T. congolense life cycle.
Microscope images of the in vitro cultivated IL3000:13–29 strain transfected with pLEW20c-EGFP vector in all the developmental stages. Non induced and tetracycline (1 µg/ml for 48 h) induced cells were fixed and visualized in phase contrast and by EGFP fluorescence (images were recorded with the same exposure time). Scale bars = 1 µm.
Figure 2.
Inducible RNAi on tubulin genes through the T. congolense life cycle.
Phase contrast microscope images of the in vitro cultivated IL3000:13–29 strain transfected with p2T7Ti/αTUB vector in all the developmental stages. Non induced and tetracycline (1 µg/ml for 24 h and 48 h) induced cells are presented. PCF, EMF colonies, MCF (after DE52 purification) and BSF on BAE feeder cell layer were observed directly in the culture medium. In the insets, PCF and MCF were fixed and stained with 4,6-diamino-2-phenylindole (DAPI) before observation in phase contrast. Scale bars = 10 µm.
Table 4.
Luciferase activity in the T. congolense pLEW20c-Rluc transfected strains.
Figure 3.
Morphological features and molecular markers analysis during metacyclogenesis.
A, Wild-type individual IL 3000 cells were labeled with monoclonal anti-PFR antibody during metacyclogenesis. For orientation the fixed cells were also stained with 4,6-diamino-2-phenylindole (DAPI) and visualized in phase contrast. Scale bar = 1 µm. B, Western blot analysis of stage specific markers during the metacyclogenesis. 106 cells of IL3000 were loaded per well. Tubulin was used as a loading control. Molecular weights are indicated on the blots side. iEMF: induced EMF in the absence of serum. Identical results were observed with the other T. congolense isolates and with transfected parasites.
Figure 4.
Infectivity of TRUM183:13–29 cell-line through mice passages.
107 in vitro differentiated MCF or a volume corresponding to 107 parasites of infected mice blood were injected in Balb-c, Balb-c pretreated with cyclophosphamide or NOD/SCID mice. Development of parasitaemia was monitored by microscopic observation of mice blood every two days during 2 months. − means that no parasite could be observed during the time period of observation, + means that parasitaemia developed during the time period of observation.
Figure 5.
Comparison of T. brucei and T. congolense in vitro growth.
A, T. brucei 427 BSF have been inoculated at 5.104 cell/ml in TcBSF-1 (Table 1) medium at 37°C in a humidified atmosphere containing 5% (vol/vol) CO2. B, T. congolense STIB910 BSF have been inoculated at 5.104 in presence of BAE (black curves) or at 2.105 cell/ml in absence of BAE (grey curves) in TcBSF-2 (Table 1) medium at 34°C in a humidified atmosphere containing 5% (vol/vol) CO2. Parasites were cultured in their specific medium for five days (——⧫) or in medium supplemented with red blood cell lysate (resuspension of red blood cells vol/vol in cold water and 100 fold diluted in medium) (——•), or with hemoglobin (100 µg/ml) (—), or in absence of reducing agents (Bathocuproin, 2-mercaptoethanol and cystein were removed) (——▪). Cells were counted every 24 h.
Figure 6.
In vitro culture system and genetic tools for T. congolense.
A, The different developmental stages cultured in vitro are represented as individual cells for PCF and MCF as adherent cells forming colonies for EMF and on BAE layer for BSF. Scale bar = 1 mm for PCF, MCF, BSF in infected mouse blood and the lower photo of BSF on BAE layer. PCF, EMF and BSF are dividing cells as represented by the rounded arrow. BSF differentiation can be achieved either by infection of mice and culture from blood or directly in vitro on BAE layer. B, Scheme of transgenic cell-line analysis through the cycle starting from PCF (top) or BSF (bottom) transfection. Linearized vectors used in transfection assays are represented by a line and two boxes, the black one represents the selection marker and the grey one represents the transgene (Tg).