Figure 1.
Percentage of CD4+ and CD8+ T cells, evaluated by flow cytometry before and after a short in vitro stimulation with T.cruzi antigen (EPI) as described in Material and Methods. A and B) CD45RAhigh expression. C and D) CD45ROhigh expression. Ex vivo = before stimulation, CC = culture non estimulated and EPI = culture after stimulation. The differences between the groups are considered significant at p less than 0.05 and are represented by * p<0.05, **p<0.01 and ***p<0.001.
Figure 2.
Cytokines secreted by naive and memory T cells.
Percentage of CD8+CD45RAhigh, CD4+CD45ROhigh and CD8+CD45ROhigh T cells, evaluated by flow cytometry after a short in vitro stimulation without or with T.cruzi antigen (EPI) as described in Material and Methods. A and B) Intracellular cytokine IFN-γ expression. C) Intracellular cytokine IL-10 expression. CC = culture non estimulated and EPI = culture after stimulation. The differences between the groups are considered significant at p less than 0.05 and are represented by * p<0.05, **p<0.01 and ***p<0.001.
Figure 3.
Analyses of central and effector memory T cells.
Percentage of CD45ROhighCCR7+ CD4+ and CD8+ T cells, evaluated by flow cytometry before and after a short in vitro stimulation with T.cruzi antigens (EPI) as described in Material and Methods. A) CD45ROhighCCR7+ expression (TCM). B) CD45ROhighCCR7− expression (TEM). Ex vivo = before stimulation, CC = culture non estimulated and EPI = culture after stimulation. The differences between the groups are considered significant at p less than 0.05 and are represented by * p<0.05, **p<0.01 and ***p<0.001.