Figure 1.
Schematic representation of EST2Secretome workflow.
EST2Secretome analysis comprising Phase I: pre-processing, assembly and conceptual translation, Phase II: identification of putative excretory-secretory (ES) proteins and Phase III: annotation of ES proteins using a suite of computational tools.
Table 1.
Nematodes parasitic in humans, other animals and plants (listed alphabetically for each host group) and their principal (definitive) hosts, selected for the analysis of excretory-secretory (ES) proteins inferred from EST data available from current databases (including dbEST).
Figure 2.
Identification and analysis of putative excretory-secretory proteins from parasitic nematode EST datasets.
The “input” EST dataset and the results obtained from each step of the workflow are shown. All of these steps, excluding two nematode-specific steps (WormHomolog and RNAi-Phenotype), are currently incorporated within EST2Secretome.
Table 2.
Summary of EST2Secretome's Phase I and Phase II results for human, other animal and plant nematode parasites.
Figure 3.
Assignment of Gene Ontology (GO) terms for putative excretory-secretory proteins.
Components, such as Biological Process, Molecular Function and Cellular Component, are indicated. Individual GO categories can have multiple mappings. Percentages shown reflect the total categories annotated and not the total sequences annotated under each component.
Table 3.
Top 25 selected metabolic pathways in excretory-secretory proteins mapped using KEGG database.
Table 4.
Top 20 nonredundant protein families of known function found in excretory-secretory proteins.
Table 5.
Identification of interaction partners: selected entries identified during the comparison and their interaction partners obtained using IntAct database.
Table 6.
Selected ES proteins with non-wild-type C. elegans RNAi phenotype.
Figure 4.
Comparison of ES proteins with the respective C. elegans, parasitic nematodes and host orthologues using SimiTri.
Data for parasitic nematodes of A. humans, B. other animals or C. plants are presented, compared with their respective host organism. The numbers at each vertex indicate the number of ES proteins matching only the specific database. The numbers on the edges indicate the number of ES proteins matching the two databases linked by that edge. The boxed number within each triangle indicates the number of ES proteins with matches to all three datasets compared: C. elegans, parasitic nematodes and host databases.
Table 7.
Example excretory-secretory proteins selected as potential drug/vaccine candidates based on literature evidence.