Table 1.
PCR patterns with DNA samples from liver abscess and stool specimens of the same ALA patients.
Figure 1.
PCR product size polymorphism in paired samples.
PCR product size polymorphism at selected STR loci using DNA samples from liver abscess (odd numbered lanes) and stool specimens (even numbered lanes) from the following amebic liver abscess patients: A. BAN-1 (1 & 2), BAN-2 (3 & 4) and BAN-3 (5 & 6). B. The Italian amebic liver abscess patient. C. BAN-4 (1 & 2), BAN-5 (3 & 4), BAN-6 (5 & 6), BAN-7 (7 & 8), BAN-8 (9 & 10), BAN-9 (11 & 12), BAN-10 (13 & 14) and BAN-11 (15 & 16). D. The USA amebic liver abscess patient. Asterisks indicate a PCR product size difference in the paired samples. A more complete set of PCR products can be seen in Figure S3.
Table 2.
Comparison between sequence types in liver abscess versus stool DNA from ALA patients.
Figure 2.
Sequence types of PCR products from paired samples.
Schematic representations of (A) locus A-L, (B) locus D-A, (C) locus STGA-D, (D) locus N-K2 and (E) locus R-R are given to illustrate the sequence type differences observed between paired samples. The asterisk indicates a single base insertion of A in the 2AL sequence. Each arrow represents a specific tRNA gene (shown inside the arrow) and colored boxes represent the STRs. The sequence types in loci N-K2, R-R, and STGA-D were according to reference 13 (EF427346-EF427363, EF421375-EF421386 and EF421387-EF421403, respectively), and locus D-A sequence types were according to Clark, Ali and Haque (EU251498–EU251501 and unpublished). 5 sequence types have been detected to date in locus A-L (EU251493–EU251497).