The circadian clock gene CYCLE as a potential target for disrupting blood-feeding behavior in the mosquito Culex pipiens
Fig 5
Mistimed blood feeding alters normal blood digestion and reproductive health.
A - Comparison of the blood digestion state in the midgut between daytime and nighttime fed mosquitoes. B - Comparison of early trypsin (eTRP) expression in midgut between daytime and nighttime fed mosquitoes. C - Comparison of late trypsin (ITRP) expression in midgut between daytime and nighttime fed mosquitoes. D - Comparison of chymotrypsin (cTRP) expression in mosquito midguts between daytime and nighttime fed mosquitoes. E - Ovarian development comparison between daytime-fed and nighttime-fed mosquitoes. F - Ovary length comparison between daytime and nighttime-fed mosquitoes (n = 15-20, per group). G - Follicle count comparison between daytime and nighttime fed mosquitoes (n = 21, per group). H - Vitellogenin (Vg) expression comparison in mosquito fat body between day-time and night-time fed mosquitoes in PBM conditions. I - Egg raft comparison between daytime and nighttime-fed mosquitoes (n = 7-12, per group). J - Egg count per raft comparison between daytime and nighttime fed mosquitoes. Student’s t-test, *P < 0.05, **P < 0.01, ****P < 0.0001. K - Linear regression analysis results for the constructed hatch rate. The hatch rate was constructed using a mean egg count for each feeding time, and an arcsine-square root transformation to compensate. PBM - Post Blood Meal. NF - Non-blood-fed. All the scale bars represent 500μM. All data are represented as Mean±SEM.