Protective and therapeutic effects of Lactobacillus brevis PQ214320 and Bacillus subtilis PQ198038 Against experimental Trichinella Infection
Fig 5
Representative histopathological photomicrographs of small intestine in the different experimental mice groups (n = 5).
C: Positive Trichinella untreated control mice showing thickening and flat of the villi (V), degeneration, atrophy, necrosis with sloughing of the upper tips of the villi (S), multiple T. spiralis larvae (arrow) in the villus cryptus junctions and at muscularis (M) associated with severe infiltration of inflammatory cells in submucosa (Sm) and lamina propria (star), hyperplasia of goblet cells (green arrow), dilatation of the blood capillary (red arrow), and edema in different intestinal layers (arrowhead) (H&E, low × 200 and high ×400 Magnifications). ALB: Infected mice treated with Albendazole, ALB group displaying sub-epithelial cell edema (arrowhead), atrophy, and sloughing (S) of the upper tips of some villi (V) with infiltration of inflammatory cells (star), muscular layer (M) (H&E, low × 200 and high ×400 Magnifications). BS: Infected mice post-treated with B. subtilis PQ198038, BS group exhibiting degeneration of the villi (V) with infiltration of inflammatory cells (star), and sub-mucosal edema (arrowhead) in-between crypts of Lieberkühn (CL), muscular layer (M) (H&E, low × 200 and high ×400 Magnifications). BSP: Infected mice pre-treated with B. subtilis PQ198038, before infection and continued after infection, BSP group displaying a sloughing (S) of the upper tips of some villi (V) with infiltration of inflammatory cells (star), degenerated crypts of Lieberkühn (blue arrow), hyperplasia of goblet cells (green arrow), and dilatation of the blood capillary (red arrow), muscular layer (M) (H&E, low × 200 and high ×400 Magnifications). L: Infected mice post-treated with L. brevis PQ214320, L group showing an improvement of villi (V) with a slight sloughing (S), crypts of Lieberkühn (CL), muscular layer (M) (H&E, low × 200 and high ×400 Magnifications). LP: Infected mice pre-treated with L. brevis PQ214320, before infection and continued after infection, LP group exhibiting a noticeable improvement with slight signs of edema (arrowhead), crypts of Lieberkühn (CL), muscular layer (M) (H&E, low × 200 and high ×400 Magnifications).