Intestinal Lactobacillus johnsonii protects against neuroangiostrongyliasis in BALB/c mice through modulation of immune response
Fig 5
Lactobacillus johnsonii inoculation alters immune pathways in mice with neuroangiostrongyliasis.
Transcriptomic analysis of the brain between A. cantonensis-infected mice with vehicle or Lactobacillus johnsonii inoculation. (A) Volcano plot of differentially expressed genes (DEGs) identified between vehicle-treated and L. johnsonii-treated, A. cantonensis-infected mice. Data are presented in log2 fold change of gene expression. Each dot represents a single gene. Grey dots represent non-significant genes, blue dots represent down-regulated DEGs, and red dots represent up-regulated DEGs. Significant DEGs are indicated as FC > 2 and FDR < 0.05. (B) Heatmap showing the 435 significant DEGs. Different color codes represent different clusters of genes with similar functions or participate in the same biological processes. Clustering was done with the Log10(FPKM + 1) values. (C) Gene ontology (GO) classifications based on significantly enriched GO terms. The x-axis shows the -log10(p-value) of each term. (D) GO classifications based on the number of DEGs in each GO category. The x-axis shows the number of DEGs. Only the top 30 most prominent GO categories are shown. Different color codes represent different GO categories. (E) KEGG enrichment histogram. The x-axis indicates the number of genes in each pathway term.