Cas10 based 7SL-sRNA diagnostic for the detection of active trypanosomosis
Fig 5
7SL-sRNA detection from in vitro T. congolense (A) and T. brucei (B) cell culture supernatants.
Fold change for Cmr/NucC fluorescence and RT-qPCR assays are shown on the left y-axis. The parasite cell count is shown on the right y-axis. Filled symbols indicate a positive test (above threshold), whereas open symbols indicate a negative test result for the Cmr/ NucC and qPCR assays. The mean and SD from triplicates are shown.