Spatial proteomics of Onchocerca volvulus with pleomorphic neoplasms shows local and systemic dysregulation of protein expression
Fig 5
Immunhistolocalization of OvDig-1 (OVOC8391) in a PN female O. volvulus.
A: Cross-section of a O. volvulus female with a few stretched Mf in the vagina, negative control using pre-immune serum. B: Same section as in A but stained (red) with an antiserum raised against OvDig-1. The protein is localized in the hypodermis, connective muscle tissue and in the neoplasm. Stretched Mf are also OvDig-1 positive (arrow). C: Cross-section of a female with PN cells in both uterus branches. The outer uterus wall, the connective tissue, muscle and PN cells are OvDig-1positive (red). D: PN in the pseudocoelom cavity with strong staining for OvDig-1. Both uterus branches are empty, constricted and not labeled. E: PN in the uterus strongly labeled for OvDig-1. PN in the pseudocoelomic cavity shows a more homogenous cell type with weak labeling. HC: healthy control, PN: pleomorphic neoplasm, i = intestine, mu= muscle, ut =uterus, scale bar= 50 μm.