The evolutionary and molecular history of a chikungunya virus outbreak lineage
Fig 2
Emergent mutations in the Nsp1-Nsp2-Nsp4 replication complex.
A) Representative view of the CHIKV replication complex. Nsp1 is in grey, Nsp2 in yellow and Nsp4 in pink colour. Structural models for visualization were obtained from PDBid 7y38 [45] and 4ztb [46]. In all panels the mutations arising in the IOL are shown as purple sticks. Note that highlighted amino acids correspond to the position of the indicated mutations but not always to the actual amino acid involved in the mutation process. (see methods for more information) B) Close-up view, highlighting the mutations on Nsp1. The GTP and ATP which are cofactors for the Nsp1 are also shown as orange sticks. C) Model of Nsp2 with indicated mutations. The catalytic residues are shown as green sticks and the RNA fragment bound to the helicase domain is shown as orange sticks. D) Detailed view showcasing the mutations on Nsp4. E) Summary of all mutations found in the replication complex.