Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division
Fig 5
The new FAZ does not reach length parity with the old FAZ prior to the completion of cell division.
(A) Cells were fixed and stained with 1B41 to label the FAZ and posterior punctum and DAPI to label DNA. The cells were then imaged using epifluorescence and DIC microscopy, followed by measurement of all the cell features depicted in this schematic. All graphs were generated as SuperPlots, where each independent experiment is shown in a separate color, with the large circles represent the mean of each independent experiment, and black lines represent the mean and the standard deviation. (B) Measurements of the length of the new FAZ as cells progress through cell division. (C) Measurements of the length of the new flagellum as cells progress through cell division. (D) Ratio of the length of the new FAZ to the old FAZ as cells progress through cell division. (E) Ratio of the length of the new flagellum to the old flagellum as cells progress through cell division. Statistical analysis was performed using one-way ANOVA ****p < 0.0001.