Long read single cell RNA sequencing reveals the isoform diversity of Plasmodium vivax transcripts
Fig 4
Example of isoforms expressed in a stage-specific manner.
(A) Each panel shows the PacBio reads mapped to the glutaredoxin 1 (PVP01_0833900) and split in four groups according to the stage of the parasites they derived from: early trophozoites, late trophozoites, schizonts and female gametocytes (the terminology used for each group reflects developmental categories based on pseudotime analysis and might not exactly correspond to stages determined by microscopy). Female gametocytes express glutaredoxin 1 from a more upstream TSS than asexual parasites, and the resulting transcripts have an additional intron in the 5’-UTR (red box). (Note also the presence on an alternatively spliced intron in the 3’UTR of some transcripts). (B) PCAs showing that the short (blue) and long (red) isoforms for glutaredoxin-1 are expressed at stages of the parasite development, with the long isoform almost exclusively expressed in female gametocytes.