Quantitative single-cell analysis of Leishmania major amastigote differentiation demonstrates variably extended expression of the lipophosphoglycan (LPG) virulence factor in different host cell types
Fig 8
Localization of T18 antibody labeling.
(A) Epifluorescent microscopy of an amastigote (72 h post metacyclic infection) within a PEM labeled with T18 antibody along with antibodies recognizing parasite histones and DNA stain Hoechst 33342. Scale bar, 2 μm. (B, C) Electron micrograph of an L. major amastigote within a PEM following immuno-gold labeling with T18 antibody. The region within the dashed box (left) is shown to the right at higher magnification in panel C. (D, E) Additional examples of the posterior end of T18 immuno-gold-labeled parasites. (F) TEM image of the posterior end of an L. major amastigote. These cells were subjected to a different fixation protocol than what was used for immunoEM (see Methods). Arrowheads in panels C-F point to the amastigote-specific structure at the posterior end of the parasite. Scale bar for EM images, 0.5 μm. Abbreviations: p, parasite posterior; n, nucleus; k, kinetoplast.