Quantitative single-cell analysis of Leishmania major amastigote differentiation demonstrates variably extended expression of the lipophosphoglycan (LPG) virulence factor in different host cell types
Fig 4
Initiation of parasite replication delayed in bone-marrow derived macrophages and DC.
(A) PEM, BMM, and BMDC were infected with metacyclic-stage parasites and cultured in the presence of BrdU for the indicated time points prior to fixation. The percent of parasites showing BrdU-positive nuclei was determined by analysis of samples stained with anti-BrdU and anti-L. major histone antibodies. N > 350 parasites per cell type/time point. (B) The number of parasites per 100 host cells at 24 and 72 h post infection of PEM, BMM, and BMDC. Data, mean ± S.E.M. of three independent experiments. *, P < 0.05, **, P < 0.001 by ANOVA. N.S., not significant.