Quantitative single-cell analysis of Leishmania major amastigote differentiation demonstrates variably extended expression of the lipophosphoglycan (LPG) virulence factor in different host cell types
Fig 3
Metacyclic-to-amastigote transition is similar in different host cell types.
(A-D) Comparison of metacyclic-to-amastigote transition in PEM (black diamonds), BMM (gray squares) and BMDC (open triangles) for the percent of parasites positive for PFR labeling (A), mAb T18 labeling (B), YFP signal (C), and mAb T17 labeling (D). (E) Comparison of parasite cell shape 24 h after infection of the indicated cell types with metacyclic-stage L. major. Data, means ± S.E.M for 3 independent experiments; *, P < 0.05; **, P < 0.001 by ANOVA; N.S., not significant. N > 350 parasites per marker/time point.