Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus
Fig 3
Immunohistochemical visualization and electron micrographs of EBIV in Ae. aegypti midgut and ovary.
Immunolocalization of EBIV antigen in midguts of mosquitoes with oral infection at 14 dpi (A) and intrathoracic inoculation at 7 dpi (B). Immunolocalization of EBIV antigen in ovaries of mosquitoes infected via intrathoracic inoculation at 7 dpi (C). Immunolocalization of EBIV antigen in midguts of mosquitoes fed blood without EBIV at 14 dpi (D) and mosquitoes injected with 100 nl RPMI 1640 at 7 dpi (E). Immunolocalization of EBIV antigen in ovaries from mock-injected mosquitoes at 7 dpi (F). F-actin was stained with phalloidin (green). The cell nucleus was stained with DAPI (blue). NC: nurse cells, FC: follicle cells. (G-J) Virions observed in gut are indicated by red arrows on electron micrographs. H and J are the enlarged insets of the boxes in G and I, respectively. (K-N) Virions observed in ovary are indicated by red arrows on electron micrographs. L and N are the enlarged insets of the boxes in K and M, respectively. EBIV virion clusters were detected using a mouse anti-EBIV polyclonal antibody and goat anti-mouse IgG labeled with red fluorescent secondary antibody.