Partial masculinization of Aedes aegypti females by conditional expression of Nix
Fig 9
Impact of doxycycline on transgenic Nix expression.
Quantification of accumulated transgenic Nix transcript treated and non-treated with 50μg/ml of Doxycycline from embryos collected 0-2h, 2-4h, 4-6h, 6-8hs after being laid from F3, F6b, and P5. (A) Transgenic lines and (B) Adult female mosquitoes. Columns represent the mean values and bars the standard deviation (A-B) of at least two independent experiments containing at least 50 embryos each (A) and 3 independent experiments (B). Multiple t test was used to determine the statistical significance (B); ns: P>0.05; ***: P<0.0001.