Partial masculinization of Aedes aegypti females by conditional expression of Nix
Fig 7
Transgenic Nix expression profile in transgenic mosquitoes.
(A) Schematic representation of the pTRE-Nix/NixPro-tTA transgene (top) and endogenous Nix gene (bottom) and Nix T (specific for the transgene) and Nix End (binds on the Nix open reading frame on both the transgene and endogenous Nix) primer binding localization. (B) Quantification of endogenous or transgenic Nix transcripts in F3, F6b and P5 transgenic adult female mosquitoes and Liverpool males of at least 3 experiments containing 5 mosquitoes each. Quantification of accumulated transcript in the maxillary palps (C, E) and antennae (D, F) from 25–30 male Liverpool or female transgenic of transgenic Nix and Nix endogenous (C-D) and tTA and transgenic Nix (E-F). Columns represent the mean values and bars the standard. One-way ANOVA test was used to determine the statistical significance; ns: P>0.05; ****: P<0.0001. T Nix, transgenic Nix transcript; Nix End, endogenous Nix transcript, tTA, tetracycline transactivator.