Partial masculinization of Aedes aegypti females by conditional expression of Nix
Fig 2
Intersex phenotypes in transgenic Ae. aegypti with Nix under the control of the TRE.
(A) Sex ratio of each transgenic line. Binomial test was used to access statistical significance (B) Representative images of the head of the seven transgenic lines, male and female Liverpool strain depicting different maxillary palp lengths, red arrow indicating the maxillary palps from male and female wild type. (C) Maxillary palp size in millimeters from female mosquitoes of the seven transgenic lines, male and female from Liverpool in the absence of Dox. Bars represent the median. (D) Body size in millimeters from female mosquitoes of the seven transgenic lines, male and female from Liverpool strain in the absence of Dox. Bars represent the median. (E) Number of females from each transgenic line in absence of Dox that developed the male genitalia with the presence of one gonostilus (F) Pictures of F3 transgenic line, male and female Liverpool strain abdomen, the arrows are pointing to the genitalia depicting the presence (male), absence (female) or the presence of only one gonostilus (F3 line). (C) and (D) Numbers followed by different letters are statistically different P≤ 0.05 (Tukey’s test).