Zika virus-like particle vaccine fusion loop mutation increases production yield but fails to protect AG129 mice against Zika virus challenge
Fig 5
Protection afforded by VLP immunization as measured by weight loss and survival. The vaccination schedule is shown in Fig 3A.
(A) Weight changes from day 4 post-challenge of F108A VLP-immunized (blue), lead VLP-immunized (red), and control [naïve challenged (naïve), alum-only immunized and challenged (alum-only), and naïve unchallenged (uninfected)] mice. Each symbol corresponds to the mean cumulative weight change on days 4 to 20 for each mouse group (n = 5), and error bars indicate standard error of the mean (SEM). Statistical comparisons of weight change per day were performed by one-way ANOVA followed by Dunnett’s multiple comparisons tests between all groups each day. Significance levels between control and VLP-immunized groups relative to the Alum-only group are indicated in S4 Fig. (B) Day 20 post-challenge weight changes were combined for F108A and lead VLP groups, respectively. Statistical significances were obtained by performing an unpaired t-test and means for each combined VLP dose groups are indicated on top. (C) Survival of mice was assessed up to 20 days post-challenge.