Evaluation of Whatman FTA cards for the preservation of yellow fever virus RNA for use in molecular diagnostics
Fig 3
FTA card stabilization of YFV RNA decrease at high temperature.
The vaccine strain of YFV, 17D-204, was diluted in PBS, spotted onto punches from FTA cards and placed at RT (~25°C) or 37°C for two weeks. The dotted line at Ct value 37 indicates the cut off for YFV RNA positivity and is based on cut-offs used in the molecular diagnosis of YFV in the field. Points represent the average of two experiments where extracts from one punch were made and were fit using a linear regression.