Deletion C-terminal thioesterase abolishes melanin biosynthesis, affects metabolism and reduces the pathogenesis of Fonsecaea monophora
Fig 2
Effects of the 500 bp C-terminal of TE on the radial growth, biomass and conidiation of F. monophora.
(A) The conidia of each strain were inoculated on PDA and incubated at 25°C and 37°C for 14 days. The radial growth rate of the Δpks1-TE-C500 strain was significantly than that of the wild-type and Complemented strain. (B) After the growth of each strain on PDA for 14 days at 25°C and 37°C, the cells were collected, dried, and weighed. The Δpks1-TE-C500 had a significantly lower biomass. (C) After the growth of each strain on PDA for 14 days, samples of fungi (1 cm2) were collected; the spores were separated, and the number of spores was counted. The Δpks1-TE-C500 had fewer spore. All statistical analysis were performed using two-tailed t-test (**, P<0.01; ***, P<0.001; ****, P<0.0001).