Targeting Echinococcus multilocularis PIM kinase for improving anti-parasitic chemotherapy
Fig 6
Effects of SGI-1776 and CX-6258 on MV and PC.
(A) Inhibitor effects on mature MV. E. multilocularis MV were incubated for 28 d in the presence of different inhibitor concentrations as indicated below (with medium and inhibitor replacement every 3–4 d), and the number of structurally intact MV was inspected microscopically. One way ANOVA followed by Dunnet’s multiple comparison test was used for comparison to the control DMSO group. ** indicates p ≤ 0.0021. (B) Representative examples of MV incubated with different concentrations of inhibitors as indicated to the left. (C) Inhibitor effectos on the formation of MV from PC. Parasite stem cell cultures were incubated for 21 d in the presence of different inhibitor concentrations as indicated below. Numbers of fully mature MV were subsequently counted. Error bars represent standard deviation. Kruskal-Wallis test followed by Dunn’s multiple comparison test was used for comparisons with control (DMSO) group. * represents p ≤ 0.0332.