A mouse infection model and long-term lymphatic endothelium co-culture system to evaluate drugs against adult Brugia malayi
Fig 6
Validation of culture model as a direct-acting macrofilaricide drug model.
(A) Schematic of macrofilaricidal drug screen (B) Kaplein-Meyer survival or (C) average motility scores of female B. malayi over 14 days during exposure to 10μM of flubendazole (FBZ), suramin (SUR) or vehicle (1% DMSO), (D) individual motility scores or (E) MTT reductase activity following 14 days of 10μM FBZ, SUR or 1% DMSO vehicle. Data is combined from two experiments with 6 culture wells initially containing 12 viable female B. malayi. Horizontal bars represent median with interquartile range. Significance is indicated ***P<0.001, **P<0.01, *P<0.05 (Kruskal-Wallis with Dunn’s multiple comparison’s tests.