A mouse infection model and long-term lymphatic endothelium co-culture system to evaluate drugs against adult Brugia malayi
Fig 3
21 day trans-well adult B. malayi female co-culture system with macrophage leukocytes or LEC bilayers.
(A) schematic of 2D trans-well co-culture system (B) Kaplein-Meyer survival curves or (C) average motility scores over 21-day culture period. (D) individual motility scores or (E) MTT reductase activity at day 21 normalized to median MTT reductase activity in a sample of five freshly isolated female B. malayi (median optical density = 0.48). Data is combined from 6 culture wells initially containing 12 viable female B. malayi. Horizontal bars represent the median with interquartile range. Significance is indicated ***P<0.001, **P<0.01, *P<0.05 (Mantel-Cox log-rank test or Kruskal-Wallis with Dunn’s multiple comparison’s tests).