A mouse infection model and long-term lymphatic endothelium co-culture system to evaluate drugs against adult Brugia malayi
Fig 2
female and male B. malayi survival, motility and metabolic activity during culture with different cell and media conditions.
(A) Kaplein-Meier survival or (B) average motility of female B. malayi over 14 days. (C) Individual female B. malayi motility scores or (D) MTT reductase activity of cultured female B. malayi at day 14, normalized to median MTT reductase activity in a sample five freshly isolated female B. malayi (median optical density = 0.48). (E) Kaplein-Meier survival or (F) average motility of male B. malayi over 14 days. (G) Individual male B. malayi motility scores or (H) MTT reductase activity of cultured male B. malayi at day 14, normalized to median MTT reductase activity in a sample five freshly isolated male B. malayi (median optical density = 0.32). Data is combined from 24 culture wells initially containing 48 viable filariae (A,E) or between 6–12 culture wells initially containing 12–24 viable filariae (B-D and F-H). Horizontal bars represent the median with interquartile range. Significance is indicated ***P<0.001, **P<0.01, *P<0.05 (Kruskal-Wallis with Dunn’s multiple comparison’s tests).